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For those who may be interested :
Photo taken in raw. Opened in Photoshop Elements 11 after minor adjustments.
In PSE 11 used Lighting - levels - ticked on black and used eye of fish as black standard.
Noise reduction in various stages to remove spots
Used unsharp mask (minimum)
Slightly increased brightness.
Nikon D 5200 Aperture mode ISO 1250 1/40 sec f 5 62 mm